Coding

Part:BBa_K2984054

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-16)


L1c-PsadIntron-bleRscp-ARS-YFP-SP20-Rbcs2

This vector is a part of the Chlamy-HUB-Collection. Level 1 construct containing secretion signal of arylsulfatase ARS fused to the flourescent protein mVenus and the SP20 glycomodule. To influence the expression of mVenus, a bleomycin resistance gene is connected through a self cleaving peptide (scp) to the part. The Promoter is the PsaDintron for enhanced expression. The construct ends with the Rbcs2 terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2051
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2051
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2051
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2051
    Illegal NgoMIV site found at 1607
    Illegal NgoMIV site found at 2528
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

colonies_total
Fig.1 - Image of a successful transformation in E. coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii.



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